Production of N-acetylglucosamine with Vibrio alginolyticus FA2, an emerging platform for economical unsterile open fermentation

Production of N-acetylglucosamine with Vibrio alginolyticus FA2, an emerging platform for economical unsterile open fermentation

Blog Article

Members of the Vibrionaceae family are predominantly fast-growing and halophilic microorganisms that have captured the attention of researchers owing to their potential applications in rapid biotechnology.Among them, Vibrio alginolyticus FA2 is a particularly noteworthy halophilic bacterium that exhibits superior growth capability.It has the potential to serve as a biotechnological platform for sustainable and eco-friendly open fermentation with seawater.

To evaluate this hypothesis, we integrated the N-acetylglucosamine (GlcNAc) pathway into V.alginolyticus FA2.Seven nag genes were knocked Double Sided Shirt out to obstruct the utilization of GlcNAc, and then 16 exogenous gna1s co-expressing with EcglmS were introduced to strengthen the flux of GlcNAc pathway, respectively.

To further enhance GlcNAc production, we fine-tuned promoter strength of the two genes and inactivated two genes alsS and alsD to prevent the production of acetoin.Furthermore, unsterile open fermentation was carried out using simulated seawater and a chemically defined medium, resulting in the production of Inline - Pucks 9.2 g/L GlcNAc in 14 h.

This is the first report for de-novo synthesizing GlcNAc with a Vibrio strain, facilitated by an unsterile open fermentation process employing seawater as a substitute for fresh water.This development establishes a basis for production of diverse valuable chemicals using Vibrio strains and provides insights into biomanufacture.